ABSTRACT
Objective To choose an appropriate coloured system and develop a method for the determination of the main phenolic in Dai Medicine -Clerodendranthus spicatus from different areas. Methods The main phenolic in Clerodendranthus spicatus from 19 areas was determined at 500 nm wavelength, with rosmarinic acid as reference and sodium nitrite-aluminum nitrate-sodium hydroxide as colour-developing system. Results Rosmarinic acid presented good linear relationship (r2=0.9993) in the concentration range of 8.792-17.584μg/ml.The average recovery was 100.73%and RSD was 0.85%.The content of the main phenolic in Clerodendranthus spicatus ranged from 1.16 % to 3.49 %. Conclusion The method is appropriate for the determination of the main phenolic in Clerodendranthus spicatus. The main phenolic content in samples from Yunnan province is relatively high.